基于16S rRNA测序的人体肠道微生物组分析中的iSeq和Miseq的比较
Yanling Wang1, Vin Tangpricha2,3, Andrew Gewirtz1
1Center for Inflammation, Immunity, & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, Georgia.
bioRxiv : the preprint server for biology
|September 2, 2025
概括
通过比较Illumina测序平台进行微生物组分析,这项研究发现,虽然具体指标有所不同,但MiSeq,MiSeqNano和iSeq可能会为16S rRNA基因测序得出类似的生物学结论.
科学领域:
- 微生物学
- 基因组学
- 生物信息学
背景情况:
- 在微生物组研究中,Illumina MiSeq平台被广泛采用为16S rRNA基因测序,以促进结果的比较.
- 由于成本和设备的可用性,可能需要采用其他测序方法,例如MiSeqNano套件或入门级iSeq测序器.
- 由于研究设计和方法的差异,这些不同平台对微生物组分析结果的影响在很大程度上仍未被描述.
研究的目的:
- 评估Illumina的MiSeq,MiSeqNano和iSeq平台的性能一致性,用于16S rRNA基因扩增序列.
- 确定不同的Illumina平台是否在微生物组分析中产生了可比的结果,重点是多样性和分类构成.
- 评估 MiSeqNano 和 iSeq 适用于微生物组研究的成本效益较高的替代品.
主要方法:
- 从饮食补充剂干预研究中的60个便样本中制备了一个单一的16S rRNA基因扩增库.
- 准备好的库在三个Illumina平台上进行了测序:MiSeq,MiSeqNano和iSeq.
- 通过分析α多样性,β多样性,分类组合和差异分类丰度来评估平台的性能.
主要成果:
- 与MiSeqNano相比,iSeq平台在α多样性和差异性丰度检测方面表现出更好的表现.
- 比iSeq平台提供了更高的分类分辨率.
- 尽管在某些指标上存在特定平台的差异,但所有三种平台在α和β多样性和整体分类结构上都显示出类似的核心生物模式.
结论:
- 在基于16S rRNA基因的微生物组研究中,Illumina的MiSeq,MiSeqNano和iSeq平台可能会产生类似的生物学结论.
- 测序平台的选择可能取决于具体的研究问题,所需的分类学分辨率以及后勤或成本考虑.
- 这些发现支持使用MiSeqNano和iSeq作为微生物组分析的可行替代品,扩大了16S rRNA基因测序的可用性.
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