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Updated: Sep 9, 2025

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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在CRISPR-Cas12a复合体中由K949A突变引起的形态变化驱动了有效的目标结合机制

Pragya Kesarwani1,2, Durai Sundar2,3,4

  • 1Regional Centre for Biotechnology, Faridabad, 121001, Haryana, India.

Current research in structural biology
|September 2, 2025
PubMed
概括

这项研究揭示了AsCas12a酶的特定突变如何增强基因组编辑稳定性. K949A突变提高了PAM变体的稳定性,减少了无意的DNA裂变,使基因更精确地编辑.

关键词:
通过CRISPR-Cas12a系统高斯加速的MD模拟基因组编辑PAM变种结构洞察力gRNA-DNA混合物

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