在Enterococcus faecium中解读菌素耐药性:基因分布,序列类型和全球遗传学分析
1Department of Laboratory Medicine, Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese Medicine, Nanjing, China.
Frontiers in microbiology
|September 2, 2025
概括
菌素耐药性基因 (van) 在Enterococcus faecium中广泛存在,特别是在人类群体和临床环境中. 监测携带vanA的ST17菌株的传播至关重要.
科学领域:
- 微生物学
- 基因组学
- 流行病学
背景情况:
- 菌是重要的机会性病原体.
- 在E. faecium中存在抗万科米辛的现象对公众健康构成重大威胁.
- 了解万科米辛耐药基因的遗传基础和传播至关重要.
研究的目的:
- 分析E. faecium中的van基因的全球流行和分布.
- 研究携带E. faecium菌株的遗传关系和流行病学特征.
- 确定最常见的范基因型和序列类型 (ST).
主要方法:
- 下载并过了3,256个E.体基因组序列,保留了2,235个.
- 通过使用blastn选了Van基因的基因组.
- 使用pubMLST配置文件确定序列类型 (ST).
主要成果:
- 在2,235个 (47.9%) 的E. faecium基因组中,有1,071个含有van基因.
- 确定了8种van基因型,其中vanA,vanB,vanD和vanM占47.6%.
- 在美国,ST17是最常见的ST,主要是从人类中分离出来的,vanA是2015年最常见的基因.
结论:
- 范基因在全球范围内广泛分布.
- 人类群体和临床环境显示出携带E. faecium的显著流行.
- 针对ST17菌株传播的监测和干预策略至关重要.
更多相关视频
08:58Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
6.8K
06:56Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli
Published on: March 24, 2023
5.8K
相关概念视频
01:30Development of Antibiotic Resistance
197
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
197
01:19Conjugation
83
Conjugation is a form of horizontal gene transfer that primarily occurs in bacteria and some archaea, promoting genetic diversity and adaptation. Bacteria can acquire resistance genes through conjugative plasmids, allowing them to survive antibiotic treatments that would otherwise be lethal. This process involves direct contact between cells through specialized structures such as the sex pilus and is mediated by conjugative plasmids, including the F (fertility) factor.Conjugation requires...
83
01:29Modern Molecular Taxonomy
136
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
136
00:46Genomic DNA in Prokaryotes
44.5K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
44.5K