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Updated: Jun 17, 2026

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
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在HeLa细胞的耐洗剂膜内,flotillin-2相互作用的定量蛋白质学数据

Anita Hryniewicz-Jankowska1, Michał Tracz2, Ilona Opiełka1

  • 1Department of Cytobiochemistry, Faculty of Biotechnology, University of Wrocław, ul. Joliot-Curie 14a, 50-383, Wrocław, Poland.

Data in brief
|September 2, 2025
PubMed
概括

使用BioID近距离标记绘制了膜中的flotillin-2蛋白相互作用. 这项研究确定了新的flotillin-2相互作用体,揭示了它们在信号转导和膜生物发生中的作用,这对于理解与癌症相关的信号通路至关重要.

关键词:
生物标识近距离相互作用其他其他类型一个LC-MS/MS没有标签的定量蛋白质组分析

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Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...