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双电位自我增强的电化学发光免疫传感器,用于同时检测水中的雌激素E2和E3

Romana Manzoor¹, Aniqa Sehrish², Tiantian Kong¹

¹College of Chemistry and Environmental Engineering, Institute for Advanced Study, School of Biomedical Engineering, Shenzhen University, Shenzhen, Guangdong, 518060, PR China.

|September 2, 2025

概括

开发了一种新的双电位电化学发光 (ECL) 免疫传感器,用于在水环境中同时检测17β-雌二醇 (E2) 和二醇 (E3). 这种传感器可以对这些雌激素污染物进行超灵敏的监测,

科学领域:

环境科学
分析化学
生物技术

背景情况:

由于内分泌干扰,17β-雌激醇 (E2) 和雌醇 (E3) 等天然雌激素对水生生态系统和人类健康构成重大风险.
同时监测结构相似的雌激素污染物在微量水平是具有挑战性的,但迫切需要的.

研究的目的:

在水生环境中开发一种敏感且同时检测E2和E3的方法.
为多重污染物分析创建一个强大的免疫传感器平台.

主要方法:

使用PNA-MoS₂@AuAgNCs作为ECL发射器的双电位电化学发光 (ECL) 免疫传感器的开发.
使用rGO/NiCo₂O₄空心微球作为催化放大平台,增强抗体与抗原的结合.
在不同的潜在触发接口上采用竞争性免疫反应策略进行非干扰性并行检测E2和E3.

主要成果:

实现了E2和E3的超灵敏量化,检测范围广泛 (0.0005-1500 ng/mL).
计算的检测极限为E2的0. 207 fg/ mL和E3的0. 011 pg/ mL.
已证明在不同的电位 (E2 -0.9V,E3 -1.5V) 上具有良好的ECL信号分辨率.

结论:

拟议的ECL免疫传感器为多目标雌激素检测提供了强大的多功能策略.
这项技术为复杂环境样本中的多重污染物分析提供了有前途的工具.
该传感器有效地解决了对结构相似的雌激素污染物的同时监测的需求.

关键词:

17β-雌二醇电化学发光免疫传感器环境监测埃斯醇 PNA-MoS(2) 纳米复合材料 rGO/NiCo2O4纳米颗粒

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