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新型蓝藻菌株调节Lemna trisulca的形态生理特征:从短期和长期相互作用的洞察

Ariel Kaminski¹, Bartosz Lelito², Manthos Panou³

¹Jagiellonian University, Faculty of Biochemistry, Biophysics and Biotechnology, Laboratory of Metabolomics, Gronostajowa 7 St., 30-387 Krakow, Poland; Aristotle University of Thessaloniki, School of Biology, Department of Botany, GR-541 24 Thessaloniki, Greece.

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概括

测试了五种蓝藻菌株对草植物Lemna trisulca的影响. 一些菌株改变了植物生理和超结构,其中Komarekiella chia和Nodularia mediterranea增加了蛋白质含量,但没有产生常见的毒素.

科学领域:

生理学
植物生理学
生物技术

背景情况:

菌产生各种生物活性二次代谢物,具有潜在的应用.
了解植物与菌的相互作用对于利用这些化合物至关重要.
研究了包括新物种在内的五种蓝藻菌株对Lemna trisulca的影响.

研究的目的:

评估五种蓝藻菌株对Lemna trisulca的生理和超结构影响.
评估这些菌株的新生物活性代谢物产生潜力.
在短期和长期暴露下调查植物-蓝藻相互作用.

主要方法:

Lemna trisulca暴露于菌提取物 (短期) 和共同培养 (长期).
测量了生理参数 (光合作用,呼吸,生物质,蛋白质,叶绿素).
进行了超结构分析和反应性氧物种成像.
使用HPLC/MS-MS检测常见的毒素.

主要成果:

短期暴露抑制光合作用和增加呼吸;长期共同培养通常抑制生物质积累.
在 Jaaginema sp 观察到质体的超结构变化. 这种虫有很多种.
植物蛋白含量显著增加 (33-44%).
除了Iphianassa zackieohae外,大多数菌株的含量都下降了.
没有检测到常见的毒素 (阿纳托克辛- a,圆柱,微囊素- LR).

结论:

蓝藻菌株对Lemna trisulca的生理和超结构有不同的影响.
科马雷基拉和Nodularia mediterranea显示可能增加植物蛋白质含量.
需要进一步的研究来分离新的生物活性代谢物,并了解植物与菌的相互作用.

关键词:

一个模型巨生物生物活性化合物生态毒理学光合作用超结构分析青菌

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