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Brigandì Elena¹, Bacci Barbara¹, Brunetti Barbara¹

¹Department of Veterinary Medical Sciences (DIMEVET), University of Bologna, Ozzano dell'Emilia, Italy.

Veterinary and comparative oncology

|September 2, 2025

概括

评估犬类瘤扩散需要标准化的线粒细胞计数区域 (MC). 这项研究绘制了黑色素瘤,巨细胞瘤,乳腺癌和肉瘤中的Ki67热点,以指导MC评估以获得更好的预后.

科学领域:

兽医病理学
癌症学
癌症生物学

背景情况:

线粒细胞计数 (MC) 是狗癌症的一个关键预后指标.
标准化MC评估改善了形态和计数区域的协议.
对于MC评估的瘤区域选择缺乏标准化,影响预后准确性.

研究的目的:

用 Ki67 评估狗瘤中增殖区域的空间分布.
确定线粒细胞计数 (MC) 评估的最佳区域.
与特定的犬类瘤相关联.

主要方法:

Ki67免疫组织化学被用来绘制狗瘤的扩散热点.
图像分析产生了Ki67密度图.
根据扩散模式 (焦点/多焦点,扩散) 和位置 (中心,外围,分散) 分类瘤区域.

主要成果:

92. 6% 的瘤显示多焦点扩散热点.
在黑色素瘤 (76. 7%) 和皮肤巨细胞瘤 (cMCTs) (73. 3%) 中,外围热点很常见.
软组织瘤 (STS) 经常显示分散的热点 (60.9%),而狗乳腺癌 (CMC) 则显示更多的中心热点 (16.7%).

结论:

狗瘤的扩散模式在不同类型之间有很大差异.
建议在黑色素瘤和cMCT中对MC进行外围评估.
建议对STS进行综合评估,考虑CMC的中部和边缘地区.

关键词:

没有.密度地图一个热点图像分析线粒细胞计数

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