铁伦树 (Hyla sarda) 的染色体层次的基因组组
Josephine R Paris1,2, Roberta Bisconti3, Andrea Chiocchio4
1Department of Health, Life and Environmental Sciences, University of L'Aquila, Coppito, Italy.
Scientific data
|September 2, 2025
概括
创建了一种高质量的铁伦树 (Hyla sarda) 基因组组. 这种资源有助于了解其进化范围扩张期间的表型变化的遗传基础.
科学领域:
- 基因组学
- 进化生物学
- 两动物研究
背景情况:
- 泰勒尼亚树 (Hyla sarda) 呈现出与范围扩大相关的表型变异.
- 由于缺乏参考基因组,对这种变异的遗传基础的理解受到限制.
研究的目的:
- 组装一个染色体水平的参考基因组为Hyla sarda.
- 为研究进化和保护提供基因组资源.
主要方法:
- 使用PacBio HiFi长读数,生物纳米光学地图和Hi-C数据进行基因组组.
- 用于基因组组装和基因注释的标准生物信息管道.
主要成果:
- 产生了4. 15Gb的染色体级基因组组合和13个染色体.
- 获得了高完整度得分 (BUSCO 94. 60%,k- mer 98. 30%) 和22847个注释的蛋白质编码基因.
- 重复的元素约占组装的基因组的75%.
结论:
- 高质量的Hyla sarda基因组组合是进化和保护基因组学的关键资源.
- 在冰川驱动范围转移期间研究基因型-表型相关性.
- 能够为Hyla sarda的保护工作提供未来的人口基因组学研究.
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