开发基于纸质的致病微生物电化学发光试卷
Yao Qin1,2, Shichao Yuan3, Xihan Wang2,4
1Guangdong Medical University, Dongguan, 523520, China.
BMC infectious diseases
|September 2, 2025
概括
一个新的基于纸质的测试带结合了电化学发光和侧流免疫测试,以快速检测病原体. 这种方法提供了一个快速,准确,低成本的替代点的护理测试.
科学领域:
- 生物传感器
- 分析化学
- 免疫技术
背景情况:
- 开发一种基于纸的新型电化学发光测试带 (ECL-TS).
- 侧流免疫染色学 (LFIC) 与电化学发光 (ECL) 的整合,以加强检测.
- 专注于检测致病微生物.
研究的目的:
- 创建一个便携式,快速和灵敏的病原微生物检测方法.
- 评估开发的ECL-TS的性能.
- 将该方法与核酸检测等现有检测技术进行比较.
主要方法:
- 使用双抗体三明治原理与标记和捕获单克隆抗体.
- 用于检测区域的抗体流和结合.
- 用于ECL信号生成和量化的循环电量测量.
主要成果:
- 在5分钟内检测到低检测极限 (7.96 pg/mL).
- 显示出良好的可重复性 (5.62%的偏差) 和稳定性 (6.34%的偏差).
- 对目标病毒具有高特异性,KAPPA值为0. 88.
结论:
- 基于纸质的ECL-TS是一种可行的便携式,快速和敏感的诊断工具.
- 与使用SARS-CoV-2作为模型的核酸方法相比,在速度,准确性和成本效益方面表现出优势.
- 作为各种致病微生物的临床检测工具的潜在应用.
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