患有多药耐药细菌殖民和感染的重症患者的肠道微生物和全身免疫功能障碍
Zongxin Ling1,2, Wenwen Ding3, Xia Liu4
1State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, China-Singapore Belt and Road Joint Laboratory On Infection Research and Drug Development, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, China. lingzongxin@zju.edu.cn.
多种耐药生物体 (MDRO) 的殖民和感染导致肠道功能障碍和免疫功能障碍. 针对肠道微生物群可能为抗生素耐药性 (AMR) 提供新的策略.
科学领域:
- 微生物学
- 免疫学
- 遗传学
背景情况:
- 抗菌素耐药性 (AMR) 是一个全球性的健康危机,尤其影响了患有多种耐药性生物体 (MDRO) 感染的重症患者.
- 肠道微生物群在MDRO殖民和感染中的作用很重要,但尚未完全理解.
- 在MDRO的背景下,宿主免疫反应需要进一步调查.
研究的目的:
- 在患有MDRO殖民或感染的患者中调查肠道微生物群体结构和宿主免疫反应.
- 确定与MDRO状态相关的特定微生物特征.
- 在MDRO相关疾病中探索肠道微生物群与免疫功能障碍之间的相互作用.
主要方法:
- 一项病例对照研究,涉及88名MDRO感染患者,100名MDRO殖民患者和86名健康对照患者.
- 用16S rRNA基因测序来分析肠道微生物的组成.
- 进行血清细胞因子分析以评估宿主免疫状况.
主要成果:
- MDRO队列显示微生物多样性减少和社区结构发生变化,有益细菌减少,病原体如Enterococcus和Klebsiella增加.
- 在被殖民和感染的患者中观察到肠道肠类型的转变,从细菌主导到肠球菌主导.
- 显而易见的免疫功能障碍,与抗炎标志物相关的有益细菌和具有促炎细胞因子的病原体相关.
结论:
- MDRO殖民和感染诱导显著的肠道功能障碍和免疫功能障碍.
- 微生物群与免疫相互作用对MDRO相关疾病的进展至关重要.
- 肠道微生物群是对抗抗菌素耐药性的新诊断和治疗方法的有希望的目标.
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