在CRISPR之外的Cas9 - SUMOylation,效应器类潜力和病原性适应
1Faculty of Engineering and Natural Sciences, Sabancı University, Istanbul, Turkey.
The FEBS journal
|September 3, 2025
概括
CRISPR/Cas9基因编辑工具可能在宿主细胞中具有未被识别的效应器功能,可能由宿主后翻译修饰 (PTM) 调节. 这表明Cas9可能会影响宿主-病原体相互作用和微生物毒性.
科学领域:
- 分子生物学
- 微生物致病性
- 基因编辑技术
背景情况:
- CRISPR/Cas9系统是一个强大的基因编辑工具,但其在真核细胞中的调节尚未完全理解.
- 转化后的修改 (PTM) 对真核生物中的蛋白质功能和调节至关重要.
- SUMOylation是一种PTM,可以在感染期间修改细菌和病毒效应蛋白.
研究的目的:
- 探索Cas9在宿主细胞中具有未被识别的效应器类功能的假设.
- 调查宿主介导的PTMs在调节Cas9活性中的潜在作用,特别是在lysine848中的SUMOylation.
- 考虑Cas9作为宿主调节因子对微生物毒性和宿主病原体相互作用的影响.
主要方法:
- 这是一篇观点文章,提出了一个假设,并探索现有文献.
- 基于已知的PTM机制和细菌效应蛋白的Cas9功能的推测分析.
- 关于Cas9变种的致病细菌潜在的进化适应性的讨论.
主要成果:
- 卡斯9可能具有超出其正规CRISPR免疫作用的效应器功能.
- 素848的SUMOylation被强调为一个潜在的重要,功能相关的修改.
- 病原细菌可能会进化出Cas9变体,利用宿主PTM机器产生毒性.
结论:
- 这需要系统地绘制地图和功能性调查.
- 了解Cas9 PTM可以深入了解微生物策略和宿主-病原体共同进化.
- 鉴定Cas9 PTM对于提高基于CRISPR的治疗方法的精度和安全性至关重要.
相关概念视频
CRISPR/Cas9 Genome Editing
211
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
211
CRISPR
52.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.9K
CRISPR and crRNAs
17.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
Homologous Recombination
51.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
51.5K
The Antiviral System of Bacteria and Archaea: CRISPR
123
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
123
Transduction
98
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
98


