在Xenopus laevis中肝增殖器激活受体 (PPAR) 表达的发育模式和转化高潮期间对药物激活剂的反应
Anna Bushong1,2,3, Tyler D Hoskins3, Meredith Scherer3
1Odum School of Ecology, University of Georgia, Athens, Georgia, USA.
概括
非洲爪 (Xenopus laevis) 幼虫在变形过程中表现出动态的过氧体增殖器激活受体 (PPAR). 药物PPAR激活剂没有改变基因表达,这表明X. laevis可能对外来PPAR干扰不敏感.
科学领域:
- 内分泌学
- 发育生物学
- 毒理学
背景情况:
- 过氧体增殖器激活受体 (PPAR) 对脊椎动物的新陈代谢平衡至关重要.
- 两动物,如Xenopus laevis,容易受到内分泌干扰化合物 (EDC) 的影响,这些化合物会破坏脂质代谢.
- 在Xenopus laevis幼虫发育和变形过程中,PPAR表达模式基本上没有特征.
研究的目的:
- 在晚期Xenopus laevis转化过程中描述xPPARα,xPPARβ和xPPARγ亚型的表达.
- 研究药物PPAR激动剂对Xenopus laevis的xPPAR基因表达和顶端终点的影响.
- 评估Xenopus laevis的敏感性,作为研究异生菌的PPAR介导作用的模型.
主要方法:
- 在不同发育阶段对xPPARα/β/γ基因表达的定量分析 (NF 51-66).
- 对 Xenopus laevis 幼虫暴露在 PPAR 激动剂 (皮里尼克酸,贝扎菲布拉特,西普罗菲布拉特) 中.
- 测量顶端终点,包括体重,量化质量指数 (SMI) 和相对肝脏质量.
主要成果:
- 在晚期转化过程中,xPPARα和xPPARγ呈现出动态肝表达,但有显著的例外.
- 只有在转变性高潮结束时,xPPARβ表达得到了上调 (NF 66).
- PPAR激动剂没有改变基因表达;皮里尼克酸降低了女性的身体状况.
结论:
- 在Xenopus laevis转化过程中,xPPAR亚型的动态肝表达表明它们在协调能量流中的作用.
- 缺乏对高剂量的PPAR激活剂的反应表明,Xenopus laevis作为一种敏感模型对异生PPAR干扰研究的潜在局限性.
- 需要进一步的研究,以充分了解环境污染物暴露的两动物的PPAR介导作用.
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