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Updated: May 10, 2026

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice
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在3D仿生支架上生产胰岛素的INS-1细胞培养

Mikhail Parker1, Nataraja Sekhar Yadavalli2, Kristina Peranidze1,3

  • 1Nanostructured Materials Laboratory, University of Georgia, Athens, GA, 30602, USA. sminko@uga.edu.

Journal of materials chemistry. B
|September 3, 2025
PubMed
概括

研究人员开发了用于培养产生胰岛素的INS-1细胞的新型3D多元杆. 这些支架支持细胞生长和胰岛素生产,为组织工程应用创造理想的细胞板.

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Production of Pharmaceuticals01:30

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Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...