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相关概念视频

Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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使用基于 perfluorocarbon 的液体通风与用于深层组织成像的组织清除的图像深度比较

Pascal Detampel1,2, Wolf Heusermann3, Katarzyna M Wojcik4

  • 1Department of Cell Biology and Anatomy, University of Calgary, Calgary, Alberta, Canada.

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使用完碳 (PFC) 进行部分液体通风并没有改善肺部成像深度,但使得纳米颗粒可用于观察肺上皮的相互作用. 这促进了吸入颗粒和药物输送的研究.

关键词:
在体内血管内肺部成像小鼠多光子显微镜提供纳米粒子化碳折射率组织光学清除

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科学领域:

  • 肺部生理学
  • 生物医学光学
  • 纳米技术

背景情况:

  • 血管内肺部成像对于研究气泡中纳米颗粒沉积至关重要,这与空气污染和药物输送有关.
  • 光学成像深度受到空气 - 组织界面的折射率 (RI) 不匹配的限制.

研究的目的:

  • 研究改善肺部深度光学成像的策略.
  • 评估部分液体通风和光学组织清除用于增强肺部成像和纳米粒子输送的有效性.

主要方法:

  • 用氧化碳 (PFC) 评估部分液体通风以消除RI不匹配.
  • 通过RI匹配和脂质去除评估出体光学组织清除.
  • 在体内观察到的纳米粒子输送和相互作用.

主要成果:

  • 使用PFC进行部分液体通风并没有提高成像深度.
  • 只有在去除像肺表面活性剂这样的分散脂质后,ex vivo组织清除才能提高成像深度.
  • 在体内PFC通风使同质的纳米粒子传递和实时观察肺表皮相互作用成为可能.

结论:

  • 单独消除RI不匹配不会显著改善体内肺部成像深度.
  • 用PFC进行部分液体通风,便于纳米粒子输送,用于研究肺生理和优化吸入疗法.
  • 这种技术为吸入的粒子动态实时观测提供了新的可能性.