酸盐中毒:死后血液中酸盐和酸盐水平的量化
D Schmitz1, P Stach1, M Juebner1
1Department of Toxicology, Institute of Legal Medicine, University of Cologne, Faculty of Medicine and University Hospital, Cologne, Germany.
Journal of analytical toxicology
|September 3, 2025
概括
化物过量是全球自杀的方法. 一种新的HPLC-DAD方法量化死后血液中的酸盐和酸盐,通过分析血液水平和其他法医证据来帮助诊断中毒.
科学领域:
- 法医毒理学
- 分析化学
背景情况:
- 化物 (盐) 过量是全球新兴的自杀方法.
- 在死后样本中精确量化酸盐和酸盐对于法医调查至关重要.
研究的目的:
- 开发和验证使用二极管阵列检测 (HPLC-DAD) 的高性能液体染色法,用于死后全血中酸盐和酸盐的量化.
- 确定死后血液中生理酸盐和酸盐水平的参考范围.
- 为疑似中毒病例中酸盐和酸盐度的解释提供洞察力.
主要方法:
- 开发和验证一个HPLC-DAD方法.
- 采样准备包括沉,过和液体-液体提取.
- 使用铁化稳定酸盐并通过格里斯反应量化.
主要成果:
- 疑似中毒病例中的酸盐度在1. 0-529微克/毫升 (股骨) 和1. 3-176微克/毫升 (心脏) 之间.
- 酸盐度在57 - 997微克/毫升 (股骨) 和54 - 907微克/毫升 (心脏) 之间.
- 生理上的酸盐含量高达72μg/ ml,而生理上的酸盐是无法检测的 (LOD/ LOQ:1μg/ ml).
结论:
- 开发的HPLC-DAD方法有效地量化死后血液中的酸盐和酸盐.
- 酸盐中毒的诊断可以根据检测到的酸盐或升高的酸盐水平进行.
- 综合分析结果与毒理学数据,间接证据和形态发现对于全面的法医解释至关重要.
相关概念视频
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The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by...
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