针对急性下呼吸道感染进行微生物诊断的下一代测序的评估
Fengzhen Yang1, Lihua Jiang1, Qingmei Cao1
1Department of Laboratory Medicine, Qingdao University Affiliated Yantai Yuhuangding Hospital, Yantai, China.
Frontiers in microbiology
|September 3, 2025
概括
与传统方法相比,针对性下一代测序 (tNGS) 对急性下呼吸道感染提供了更好的病原体检测. 这种先进的技术更快,更具成本效益,
科学领域:
- 微生物学
- 传染性疾病
- 基因组学
背景情况:
- 急性下呼吸道感染 (ALRI) 构成了全球重大健康挑战.
- 准确和快速的病原体识别对于有效的临床管理至关重要.
- 传统的微生物学测试 (CMT) 在灵敏度和检测时间方面存在限制.
研究的目的:
- 评估针对性下一代测序 (tNGS) 在ALRI患者中检测病原体的诊断性能.
- 将tNGS与CMT的灵敏度,及时性和成本效益进行比较.
主要方法:
- 一项涉及968名ALRI患者的回顾性研究.
- 使用tNGS和CMT (培养,染色,PCR,RT-PCR) 分析了液或支气管洗样本.
- 在这两种方法中记录了检测时间和成本.
主要成果:
- tNGS发现了更多的细菌,真菌和病毒病原体,包括* Mycoplasma pneumoniae* 和* Chlamydia psittaci* 等特定物种.
- 与CMT相比,tNGS的阳性检测率明显较高.
- tNGS与特定的分子测试 (例如,A型流感,COVID-19) 具有很高的一致性,并且在检测时间和成本方面具有优势.
结论:
- 在ALRI中检测广泛的病原体时,tNGS具有较高的灵敏度.
- 这种方法及时且具有成本效益,为传统诊断提供了有希望的替代方案.
- tNGS可以帮助指导呼吸道感染的临床诊断和治疗决策.
相关概念视频
01:29Modern Molecular Taxonomy
136
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
136
01:20Sputum Studies II: Culture and Sensitivity
580
Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
580
01:30Pneumonia III: Complications and Assessment
432
Pneumonia poses the potential for numerous complications that warrant consideration. These complications include the following:
432