改善绝对微生物细胞计数的测量质量指标
Kirsten Parratt1, David Newton2, Joy Dunkers1
1Biosystems and Biomaterials Division, National Institute of Standards and Technology, Gaithersburg, MD, United States.
Frontiers in microbiology
|September 3, 2025
概括
准确的微生物细胞计数对于各种应用至关重要. 本研究引入了基于ISO标准的框架来评估和比较不同的细胞计数方法,有助于选择最合适的技术.
科学领域:
- 微生物学
- 生物技术
- 分析化学
背景情况:
- 准确的微生物细胞量化对于生物治疗产品,食品安全和益生菌至关重要.
- 目前的方法如殖民地形成单元 (CFU),流细胞测量和聚合酶链反应 (PCR) 测量细胞的不同方面,导致非可比结果.
- 缺乏基准材料阻碍了细胞计数方法的准确性评估.
研究的目的:
- 根据ISO 20391-2:2019标准开发和展示微生物细胞计数方法的修改分析.
- 评估微生物样本的不同细胞计数技术的性能和可比性.
- 为选择适合目的的细胞计数方法提供框架.
主要方法:
- 对微生物细胞样本进行了修改后的ISO 20391-2:2019标准分析.
- 使用殖民地形成单位 (CFU),库尔特原理,光流细胞计和阻抗流细胞计量大肠杆菌样本.
- 为每个方法计算质量指标,以评估可重复性,比例性和可变性.
主要成果:
- 开发的质量指标可以在复制实验中重复.
- 在测试的细胞计数方法中观察到比例性和可变性的显著差异.
- 总细胞计数显示出良好的一致性,而可活细胞计数显示出更大的变异性.
结论:
- 引入的框架和质量指标有助于了解微生物细胞量化方法的性能.
- 结果的解释可以指导适合目的的计数方法的选择和优化.
- 这种方法支持研究人员选择适合微生物细胞计数精度的各种应用方法.
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