作为对ursodeoxycholic acid耐药的初级胆道胆炎患者的预测生物标志物
Shi-Da Pan1,2, Chu-Yue Xiong1,3, Ying-Juan Shen1
1Senior Department of Infectious Diseases, The Fifth Medical Center of PLA General Hospital, Beijing 100039, China.
血微RNAs (miRNAs) 可以预测一次性胆道胆炎 (PBC) 的治疗反应. 在PBC患者中,MiR- 126-3p具有预测ursodeoxycholic acid (UDCA) 治疗次优反应的潜力.
科学领域:
- 在肝病学中发现生物标志物
- 自免疫性肝脏疾病的分子诊断
- 在治疗反应预测中进行微RNA分析
背景情况:
- 甲酸 (UDCA) 是初级胆道胆炎 (PBC) 的标准治疗方法.
- 很大一部分PBC患者对UDCA反应不佳.
- 血微RNAs (miRNAs) 参与了PBC的发病,但缺乏预测UDCA疗效的生物标志物.
研究的目的:
- 根据患者对UDCA的反应,识别PBC患者的不同表达的miRNA.
- 评估这些miRNA作为PBC中UDCA治疗结果的预测生物标志物的潜力.
主要方法:
- 从未接受过治疗的PBC患者的血样本使用下一代测序和定量实时PCR进行分析.
- 根据巴黎I标准,患者分为药物敏感 (DS) 和耐药组.
- 为了确定预测标志物,研究小组对待前的miRNA概况进行了比较.
主要成果:
- 在治疗前,UDCA反应和不反应的PBC患者之间有49个miRNA的差异表达.
- 治疗前升高的miR-126- 3p水平与药物敏感性相关 (P < 0. 001).
- 后勤回归发现miR-126- 3p是UDCA反应的显著预测因子 (OR=34. 32, P=0. 016) 具有很高的诊断准确性 (AUC=0. 891).
结论:
- 血miRNA表达特征在PBC患者之间存在差异,UDCA治疗反应不同.
- 在PBC患者中预测低于最佳的UDCA反应的有希望的生物标志物.
- 这一发现有助于个性化PBC治疗策略.
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