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加勒-3 诱导神经发育的基极性,并调节化

Luana Campos Soares^1,2,3,4, Ning Huang^1,5, Hana Bernhardova¹

¹Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3QX, UK.

Science advances

|September 3, 2025

概括

通过维持神经干细胞的基极性 (ABP),加勒-3 (Gal-3) 对大脑发育至关重要. 阻断Gal-3会破坏ABP,影响神经发育和大脑结构.

科学领域:

神经科学
发育生物学
细胞生物学

背景情况:

基极性 (ABP) 对于正常的大脑发育至关重要.
在神经发育过程中控制ABP的精确机制尚未完全理解.
之前已经与上皮细胞中的ABP有关.

研究的目的:

在人类胚胎干细胞 (hESCs) 神经发育过程中,研究 Galectin-3 (Gal-3) 在建立和维持基极性 (ABP) 的作用.
探索Gal-3阻塞对神经红形成和基因表达的影响.
评估Gal-3调节对胚胎大脑发育的体内影响.

主要方法:

在神经诱导过程中进行免疫光染色以评估hESC中的Gal-3的顶峰表达.
在hESC衍生的神经上进行Gal-3阻塞和救援的功能性测试.
转录组分析 (RNA测序) 来识别Gal-3封锁后的基因表达变化.
在小鼠胚胎中进行体内研究,以评估Gal-3阻塞对皮质发育和转化的影响.

主要成果:

在神经诱导过程中,人类胚胎干细胞 (hESCs) 中表达了Gal-3 (Gal-3).
阻断Gal-3会破坏基极性 (ABP) 并改变hESC衍生的神经红中的连接蛋白分布,通过添加复合Gal-3可逆效应.
甲基-3 阻断改变了神经系统发育和细胞结合组合中的基因表达.
在体内,胚胎发育中的Gal-3阻断导致水平细胞分裂减少,皮质前代层层分化受损,并诱导化.

结论:

在神经发育过程中,加勒-3 (Gal-3) 在调节基极性 (ABP) 中起着至关重要的作用.
这些发现揭示了ABP在发育中的新型调节机制.
由于对胚胎大脑发育的重大影响,孕期调节Gal-3水平需要谨慎.

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