相关实验视频

Updated: Sep 9, 2025

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
09:40

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

Published on: September 23, 2011

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hnRNPL-CstF64复合物:通过ERNA和NHEJ调节,协调IgH位点重组动态中的CSR和LSR

Farazul Haque1, Mikiyo Nakata1, Hidetaka Kosako2

  • 1Department of Immunology and Genomic Medicine, Centre for Cancer Immunotherapy and Immunobiology, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan.

Nucleic acids research
|September 3, 2025
PubMed
概括

异质核蛋白L (hnRNPL) 通过形成增强器RNA转录和DNA修复的复合物来调节抗体多样化. 这一发现将RNA转录与DNA修复在类交换重组和位置自杀重组之间联系起来.

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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

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Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells12:04

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

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Homologous Recombination

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Conservative Site-specific Recombination and Phase Variation

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Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
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The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
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