评估特定单克隆抗体与固定SARS-CoV-2核蛋白之间的相互作用动力
Yana Karnitskaya1, Maryia Drobysh1, Almira Ramanaviciene2
1Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko St. 24, LT-03225 Vilnius, Lithuania.
Bioelectrochemistry (Amsterdam, Netherlands)
|September 3, 2025
概括
这项研究开发了一种电化学生物传感器来评估抗体与抗原的相互作用. 该生物传感器对特征SARS-CoV-2核蛋白和单克隆抗体相互作用具有很高的亲和力和敏感性.
科学领域:
- 生物医学工程
- 分析化学
- 免疫技术
背景情况:
- 严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 感染对全球健康构成挑战.
- SARS-CoV-2 核体蛋白 (N-蛋白) 是抗体发展的关键免疫性点.
- 对新型抗体与抗原相互作用的准确评估对于诊断和治疗至关重要.
研究的目的:
- 开发和描述一种用于评估抗体-抗原相互作用的电化学生物传感系统.
- 确定新设计的单克隆抗体 (mAb-16D9) 和SARS-CoV-2重组N蛋白之间的相互作用动力.
- 评估开发的生物传感器对抗体特征的敏感性和亲和力.
主要方法:
- 在生物传感器表面的SARS-CoV-2 N-蛋白的固定.
- 使用循环电压测量分析N-蛋白和mAb-16D9之间的结合动力学.
- 量化结合亲和力 (Kc) 和检测极限 (LOD) 用于抗体表征.
主要成果:
- 电化学生物传感器成功确定了N蛋白与mAb-16D9之间的相互作用动力学.
- 观察到mAb- 16D9对N蛋白具有强大的结合亲和力 (Kc = 50. 99μg/ ml).
- 低检测极限 (4.3 × 10−4 μg/mL) 表示生物传感器的高灵敏度.
结论:
- 开发的电化学生物传感器有效地评估了抗原-抗体相互作用动力学.
- 生物传感器系统为新设计的抗体,如mAb-16D9提供了敏感的平台.
- 这项技术有可能用于基于抗体的产品的诊断和质量控制.
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