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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

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基于特征的全组织成像与亚细胞分辨率

Jinlong Lin¹, Zach Marin², Xiaoding Wang¹

¹Lyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Cecil H. and Ida Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

Cell reports methods

|September 3, 2025

概括

此摘要是机器生成的。

我们开发了多尺度清除组织的轴扫光片显微镜 (MCT-ASLM) 用于大完整组织的高分辨率成像. 这种显微镜平台将亚细胞和组织尺度连接起来,

关键词:

CP: 图像处理一个LSFM 癌症发展基于特征的清除组织成像多尺度的ASLM 神经科学

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Last Updated: Sep 9, 2025

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科学领域:

生物医学工程
显微镜
细胞生物学

背景情况:

目前的显微镜难以在大量组织中描绘罕见的生物事件.
同时实现高分辨率和厘米尺度视野存在局限性.

研究的目的:

推出一种新的显微镜平台,即MCT-ASLM,用于完整组织的高分辨率成像.
能够在多个尺度上研究层次组织和复杂的生物过程.

主要方法:

开发了多尺度清除组织的轴扫光片显微镜 (MCT-ASLM).
该平台将厘米尺度的成像与高分辨率的目标查询 (低至300nm) 结合起来.
在人为引导或自主模式下运行,用于灵活的组织分析.

主要成果:

在微米级分辨率下,MCT-ASLM可以捕捉到21毫米的视野.
无过渡到目标成像模式,同位素分辨率接近300nm.
在绘制神经回路,可视化脏内置和检查瘤微环境方面的应用.

结论:

在全面的生物研究中,MCT-ASLM将亚细胞到组织水平的尺度相结合.
这种多功能显微镜方法有助于理解全球生物现象中的局部事件.
能够详细研究完整组织中的空间复杂的生物过程.