基于复杂化的光谱测量方法来确定贝西弗洛克萨辛:结合实验和计算见解
Abobakr A Mohamed1, Raed M Maklad2, Abdallah M Zeid3
1Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Fayoum University, 63514, Egypt.
Analytica chimica acta
|September 3, 2025
概括
一种新的光谱测量方法使用乙酸盐缓冲剂增强了化 (Bfln.HCl) 的光. 这种通过DFT计算验证的成本效益高的技术可在药物和生物样本中进行敏感的Bfln.HCl分析.
科学领域:
- 分析化学
- 药物分析
- 光谱学
背景情况:
- 光方法对药物分析具有敏感性和成本效益.
- 贝西弗洛克萨 (Bfln.HCl) 是一种治疗眼睛感染的抗生素,在酸性条件下显现光增强.
- 在酸性介质中Bfln.HCl光增强的机制需要进一步研究.
研究的目的:
- 开发一种用于增强Bfln.HCl光的新型光谱测量方法.
- 阐明Bfln.HCl光增强背后的机制.
- 在药物和生物样本中验证Bfln.HCl分析的方法.
主要方法:
- 使用乙缓冲剂 (pH4.0) 增强Bfln.HCl光的光谱测量方法的开发.
- 在添加缓冲剂时检查光变化 (波长转移,强度增加).
- 密度功能理论 (DFT) 和热力学分析的应用,以了解复杂的形成和光增强机制.
- 使用眼科制剂和人工水性幽默方法的验证.
主要成果:
- 在激发波长中观察到10纳米的红移和440纳米的光强度增加.
- DFT计算表明Bfln+与酸 (AcOH) 之间形成稳定的1:2循环复合物,与光增强相关.
- 该方法表现出高灵敏度 (LOD = 22.6 ng mL-1),线性 (100-1000 ng mL-1) 和选择性.
- 在真实样本 (眼科制剂,人工水性水分) 上成功应用,回收率高.
结论:
- 开发的光谱测量方法为Bfln.HCl的确定提供了灵敏,经济有效和环境可持续的替代方法.
- 该研究提供了Bfln.HCl光增强机制的实验和计算见解.
- 这种方法可能会扩展到分析其他基诺抗生素.
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