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开发一种新的针对性LC-MS/MS方法来确定心脏粉症的类型

Kaijuan Wang¹, Ruichen Liu², Li Li¹

¹Center of Laboratory Medicine, Beijing, Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases & Fuwai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100037, China.

Analytica chimica acta

|September 3, 2025

概括

一种新的有针对性的质谱法可以准确地识别心脏粉症 (CA) 蛋白类型,其性能优于传统方法. 这种方法为临床诊断和识别罕见的amyloidosis亚型提供了实用解决方案.

科学领域:

心血管疾病
蛋白质组学
生物标志物发现

背景情况:

在心脏粉症 (CA) 中,准确的粉蛋白类型化对于治疗和预后至关重要.
目前的组织病理学方法往往没有明确的结果.
非向性质谱测是有效的,但对于常规诊断来说成本高且耗时长.

研究的目的:

开发一种针对CA类型的新型半定量蛋白质组学方法.
克服CA现有诊断方法的局限性.

主要方法:

开发了一种使用三重四极质谱的向性半定量蛋白质组学方法.
使用激光微解剖质谱 (LMD-MS) 作为参考标准.
分析了52名CA和52名心肌缩症 (HCM) 患者的甲固定,嵌入式心肌组织.

主要成果:

关键的粉样蛋白相关蛋白 (AAP),如阿波利波蛋白E,具有很高的诊断准确性 (AUC为0. 999).
该方法精确地区分了跨氨酸粉症 (ATTR) 和免疫球蛋白轻链粉症 (AL-κ/AL-λ) 亚型.
在确定CA亚型时达到100%的准确性,显著优于免疫组织化学 (30.8%).

结论:

针对性的半定量质谱法与LMD-MS具有很高的一致性.
这种技术为临床环境中的CA类型提供了实用,准确和成本效益的替代方案.
这种方法有助于识别罕见的粉样性粉症亚型.

关键词:

心脏粉症心脏内脏活检蛋白质组学分类有针对性的LC-MS/MS

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