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Updated: Sep 9, 2025

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
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微生物全细胞生物传感器的化学信号蛋白:从设计到部署

Güniz Özer Bergman1, Sonia Mecacci2, Vitor A P Martins Dos Santos3

  • 1Bioprocess Engineering Group, Wageningen University & Research, Wageningen, 6700 AA, The Netherlands; Laboratory of Systems and Synthetic Biology, Wageningen University & Research, Wageningen, 6708, WE, The Netherlands.

Trends in biotechnology
|September 3, 2025
PubMed
概括

微生物全细胞生物传感器 (MWCB) 使用工程蛋白质,如双组件系统 (TCS) 和体转录因子 (aTF) 进行多功能分析物检测. 这篇评论探讨了在诊断,环境监测和生物制造方面的应用策略和挑战.

关键词:
化学蛋白质域名交换连接物特异性工程微生物全细胞生物传感器模块化生物传感器设计原型集成

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Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
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