原生益生菌Lactobacillus在控制炎症中的预防和治疗作用
Sara Abdollahi1, Sedigheh Mehrabian1, Mohammad Reza Pourshafie2
1Department of Microbiology, NT.C., Islamic Azad University, Tehran, Iran.
Folia microbiologica
|September 3, 2025
概括
原生乳杆菌益生菌有效降低了肠道细胞中的炎症信号通路,包括MAPK. 这些发现表明对炎症性肠病 (IBD) 和其他与炎症相关的疾病有潜在的治疗益处.
科学领域:
- 胃肠病学
- 免疫学
- 微生物学
背景情况:
- 炎症性肠病 (IBD) 是由于免疫系统失调而导致的慢性胃肠炎症.
- 益生菌可以通过影响关键信号通路来调节肠道免疫力和炎症.
研究的目的:
- 调查原生Lactobacillus spp的免疫调节作用 在MAPK和MYD88独立的炎症途径上.
- 评估特定Lactobacillus菌株在HT-29细胞中调节炎症反应的有效性.
主要方法:
- 使用在体外模型的HT-29细胞暴露于格兰阴性细菌.
- 在暴露前,暴露后和暴露期间使用五种原生乳杆菌菌株 (Lactiplantibacillus plantarum,Lacticaseibacillus rhamnosus,Levilactobacillus brevis,Limosilactobacillus reuteri) 的混合物.
- 使用定量实时PCR对MAPK和MYD88独立途径的定量基因表达.
主要成果:
- 格拉姆阴性成分暴露上调了MAPK和MYD88独立路径基因.
- 在所有治疗阶段,益生菌治疗显著降低了MAPK基因表达.
- 对益生菌治疗的MYD88独立基因表达呈现出不同的反应,表明调节.
结论:
- 原生乳杆菌种类 通过调节HT-29细胞中的MAPK和MYD88独立信号通路来证明抗炎作用.
- 测试的益生菌菌株在所有治疗阶段都表现出有益的效果,这表明它们有治疗炎症的潜力.
- 这些益生菌可用于预防和治疗IBD和其他与炎症相关的疾病.
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