在移植中快速检测急性排斥的电化学生物传感器
Rohit Gupta1,2, Nikolaos Salaris1,2, Ashish Kalkal1,2
1Nanoengineered Systems Laboratory, UCL Mechanical Engineering, University College London, London, WC1E 7JE, UK.
Advanced healthcare materials
|September 4, 2025
概括
一种新型电化学生物传感器快速检测尿液中的CXCL9和CXCL10,使得移植患者的非侵入性急性排斥 (AR) 监测成为可能. 这种点诊解决方案比传统方法提供更快的结果,改善了移植结果.
科学领域:
- 生物材料科学
- 分析化学
- 移植免疫学
背景情况:
- 接受移植的人面临急性排斥 (AR) 的高风险,这是移植损失的主要原因.
- 目前的诊断方法如活检是侵入性的,昂贵的, 缺乏实时监测能力.
- 尿中的化学物质CXCL9和CXCL10显示为AR的非侵入性生物标志物,但需要劳动密集的检测方法.
研究的目的:
- 开发一个快速,无标签的电化学生物传感平台,同时量化尿液中的CXCL9和CXCL10.
- 创建一个治疗点 (POC) 解决方案,用于对移植患者进行非侵入性急性排斥监测.
- 在临床环境中评估生物传感器的诊断准确性.
主要方法:
- 用Ti3C2TxMXene交联牛血清白蛋白水凝修改的丝印碳电极的制造.
- 在15分钟内从小尿量 (5μL) 中同时电化学检测CXCL9和CXCL10.
- 使用生物传感器数据和机器学习 (启动后勤回归) 进行AR分类的前性临床研究.
主要成果:
- 生物传感器实现了单位的pg/ mL灵敏度,达到临床值,信号稳定性为30天.
- 该平台仅根据化学激素水平对AR进行了分类,准确度达到了83%.
- 将生物传感器数据与临床和组织病理特征相结合,提高了AR分类准确率至98%.
结论:
- 开发的电化学生物传感平台提供了一个可扩展的,具有成本效益的POC解决方案,用于实时的非侵入性AR监测.
- 这项技术有可能减少对侵袭性活检的依赖,使早期干预成为可能,并改善长期移植结果.
- 综合先进的材料,生物传感器工程和机器学习在移植诊断方面取得了重大进展.
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