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人工细胞与活细胞之间基于RNA的通信的可光激活合成外体

Alexander B Cook¹, Siwen Sun¹, Yudong Li¹

¹Bio-Organic Chemistry, Departments of Biomedical Engineering and Chemical Engineering & Chemistry, Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the Netherlands.

Angewandte Chemie (International ed. in English)

|September 4, 2025

概括

研究人员利用RNA囊泡开发出与活细胞交流的人造细胞. 这一突破模仿了自然的外体,使人造细胞能够调节哺乳动物细胞中的蛋白质表达.

科学领域:

合成生物学
生物材料工程
蜂通信

背景情况:

自然细胞通过复杂的机制进行通信,包括囊泡介导的RNA等生物活性分子的转移.
人工细胞研究旨在复制细胞功能,但物种间的沟通仍然是一个挑战.
目前的人工活细胞通信主要依赖于小分子交换.

研究的目的:

设计能够与哺乳动物细胞进行RNA通信的人造细胞.
开发基于囊泡的细胞间RNA转移的模块化平台.
通过人工细胞来证明自然细胞中的蛋白质表达的调节.

主要方法:

使用基于粉的复杂协体和聚合物膜构建人工细胞.
在含有可光裂表面群的聚合体内封装RNA.
通过光触发的表面修饰诱导电荷切换和囊泡驱逐.
用人工和天然哺乳动物细胞进行共同培养实验,以评估RNA转移和基因表达调节.

主要成果:

成功组装了能够封装和释放RNA的人工细胞.
从人工细胞中释放含有RNA的光诱导.
在实验室中确认RNA从人造细胞转移到自然细胞.
展示了人工细胞通过RNA通信调节哺乳动物细胞蛋白质表达的能力.

结论:

人工细胞可以通过囊泡介导与自然细胞进行RNA通信.
这种方法模仿了自然的外体功能,提升了人造细胞的能力.
开发的系统代表了创造更像生命的人工细胞和细胞间通信系统的重要一步.

关键词:

人工电池生物材料聚合体通过RNA通信超分子系统

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