相关实验视频
Updated: Sep 9, 2025

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Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
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表皮融合是由部分表皮-介质细胞转换介导的
Varsha N Tamilkumar1,2, Harsha Purushothama1, Raj K Ladher1
1National Centre for Biological Sciences, Tata Institute for Fundamental Research, GKVK PO, Bellary Road, Bangalore, 560065, India.
Biology open
|September 4, 2025
概括
在器官发育过程中,上皮质融合依赖于经历了部分上皮质到介质细胞过渡 (EMT) 的专门细胞. 这些细胞动态地改变它们的形状和粘附,以促进适当的组织关闭和细分.
科学领域:
- 发育生物学
- 细胞生物学
- 遗传学
背景情况:
- 表面膜融合对于器官发育至关重要, 但驱动它的细胞机制尚不清楚.
- 之前的研究集中在神经管和口腔关闭上,
- 细胞转换使上皮细胞融合成为一个重要的知识差距.
研究的目的:
- 在小眼囊关闭过程中研究上皮质融合的细胞和分子机制.
- 确定和描述参与调解这种形态遗传过程的特定细胞类型.
- 了解表皮细胞到介质细胞转换 (EMT) 在表皮细胞融合中的作用.
主要方法:
- 小眼囊组织的微切割.
- 用于识别不同细胞群的转录形状.
- 用CRISPR-Cas9基因编辑来评估基因功能.
- 免疫光显微镜分析细胞形态和蛋白质定位.
主要成果:
- 在上皮界面发现了一种短暂的视角边缘 (OE) 细胞群,其极性降低,细胞结合发生变化.
- OE细胞表现出部分上皮细胞转化为介质酶细胞 (EMT) 现型的特征,包括高球性和缺乏基底接触.
- 转录组分析显示OE细胞在转录上是不同的,对EMT调节器,ECM重塑基因和WNT通路组件进行了丰富.
- 对Grhl2或Sp8转录因子的破坏破坏了细胞形态,结合完整性和上皮分离.
结论:
- 表皮融合需要一个调节的混合EMT状态,以平衡结节可塑性和组织凝聚力.
- 在形态发生过程中,具有融合能力的上皮细胞积极调节它们的形状,极性,粘附性和遗传特征.
- 这项研究为细胞上皮融合提供了新的见解, 这对于理解器官发育和先天缺陷至关重要.
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