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用于储存牛AI剂量的液储的细菌污染:一个横截面研究

Britta Hensel¹, Yesica Schulze¹, Maria Kasten¹

¹Institute for Reproduction of Farm Animals Schönow, Bernau, Germany.

Reproduction in domestic animals = Zuchthygiene

|September 4, 2025

概括

在用于公牛精子保存的液 (LN2) 容器中,细菌污染很常见. 超过80%的水箱被污染, 凸显了人工授精中心需要改善的卫生规范.

科学领域:

兽医微生物学
动物繁殖
冰冷保存技术

背景情况:

在液 (LN2) 容器中冷保存牛精液,用于人工授精 (AI).
细菌污染可能会对精子质量和活力产生负面影响.
目前对LN2卫生的监测往往是不够的.

研究的目的:

评估AI中心常用的LN2中细菌污染的发生率.
确定存在的细菌物种并评估它们对抗菌素的敏感性.

主要方法:

在现场条件下从19个AI中心采集141个LN2坦克.
细菌物种的隔离和识别.
对13种常见的抗生素进行抗菌敏感性测试.

主要成果:

发现有很高的细菌污染,其中81.7%的LN2受到影响.
污染的水箱里有1到4种不同的细菌.
发现了25个细菌属和49个不同物种.

结论:

LN2坦克的细菌污染是普遍存在的.
系统监测LN2的卫生状况至关重要.
建议实施卫生指南,以保持精液质量.

关键词:

人工授精公牛精液污染情况微生物学气精子储存

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