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冗余和独特的调节元素是Drosophila快速演变的基础

Logan A Brubaker¹, Hayley Long¹, Allison Pavlus¹

¹Department of Biology, University of Dayton, 300 College Park, Dayton, OH 45469, USA.

Molecular biology and evolution

|September 4, 2025

概括

了解基因调节网络中的 cis 调节元件进化是元动物多样性的关键. 尽管特征进化,但Drosophila腹部色素的冗余 cis-调节元素表明基因表达得到保存.

科学领域:

发育生物学
进化遗传学
分子生物学

背景情况:

对基因调节网络 (GRN) 演化和元动物多样性有着至关重要的特征.
CREs的变化与特征进化有关,但冗余的CRE架构使进化研究复杂化.
冗余的CREs引发了关于这种结构的基因如何演变表达的问题.

研究的目的:

通过使用Drosophila melanogaster的性双形腹部颜色作为模型来研究CREs的功能和演变.
验证预测的腹部CREs并分析它们在Drosophila物种中的保存情况.
了解CRE冗余在基因表达和表型特征的演变中的作用.

主要方法:

评估和验证预测的腹部颜色调节元件 (CREs).
在转录因子位点 (包括同胸部,粒状头部和Eip74EF) 中分析CRE功能和保存.
具有不同色素表型的Drosophila物种中CRE活性和基因表达的比较分析.

主要成果:

大多数预测的腹部CREs得到了验证,包括同胸部,粒状头部和Eip74EF位点.
同体胸部CREs显示出部分多余的色素功能,在物种之间保持表达和活性.
在Eip74EF中,广泛的CRE时空冗余性被保存了超过3000万年,比二态特征更早.

结论:

基因调节网络中的冗余CRE架构可以保持基因表达,尽管特征发生了进化变化.
染色体的进化变化可能有利于非冗余CREs的变化,而冗余调节的基因保持保留的表达.
这项研究提供了基因调节网络如何演变的见解,并为元动物多样性做出了贡献,特别是在冗余的调节元素的背景下.

关键词:

类植物调节元素增强剂基因调控网络模块化颜色变化裁员情况转录因子

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