维生素E通过调节节点介导的信号通路来增强免疫功能和肠道组织结构:海Apostichopus japonicus的案例研究
Zitong Wang1, Yan Wang1, Xianyu Wang1
1Key Laboratory of Mariculture and Stock Enhancement in North China's Sea (Ministry of Agriculture and Rural Affairs), Dalian Ocean University, Dalian 116023, China.
维生素E (VE) 提高了日本海的肠道健康,通过提高节点调节,促进原合成和质完整性. 节点基因倒置会损害生长和肠道结构,通过补充VE减轻影响.
科学领域:
- 水产养殖营养和生理学
- 鱼类肠道健康和组织学
- 动物生长中的分子机制
背景情况:
- 肠道组织完整性对于鱼的健康至关重要,因为它依赖于上皮细胞的排列和原的结构.
- 结节蛋白是一种TGF-β家族成员,影响细胞增殖,而原蛋白则支持细胞粘附和再生.
- 了解营养素在维持肠道健康中的作用对于水产养殖至关重要.
研究的目的:
- 调查Nodal基因在青少年日本海 (A. japonicus) 中调节肠道原合成和组织完整性的作用.
- 确定饮食中维生素E水平对节点表达,原蛋白合成和肠道健康的影响.
- 阐明通过VE影响免疫功能和肠道结构的节点依赖途径.
主要方法:
- 在21天的食试验中,使用年轻的A. japonicus和含有不同维生素E水平的饮食 (0, 200, 400 mg/kg).
- 使用RNA干扰 (RNAi) 来破坏节点基因.
- 分析生长性能,免疫酶活动,基因表达 (节点,原相关) 和肠形态.
主要成果:
- 饮食补充剂200mg/kg VE显著改善了生长,免疫参数和肠形态.
- 在200毫克/公斤上调节节点表达,激活原蛋白合成和促进原蛋白沉积.
- 节点基因被淘汰导致生长减缓,肠道损伤和原蛋白合成受损,特别是在没有VE的鱼类中.
结论:
- 维生素E通过依赖节点的途径改善了A. japonicus的免疫功能和肠道组织结构.
- 节点在维持肠道原合成和组织完整性方面起着至关重要的作用.
- 通过节点信号通路的调节,饮食补充VE对改善幼虫的肠道健康有好处.
更多相关视频
08:58Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
Published on: January 5, 2017
05:29Obtaining Hemocytes from the Hawaiian Bobtail Squid Euprymna scolopes and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria
Published on: February 11, 2010
相关概念视频
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
Role Of Notch Signalling In Intestinal Stem Cell Renewal
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Immune Response Against Viral Pathogens
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
Mucosal Barrier of the Stomach
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
Cytotoxic T Cells-mediated Immune Response
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
