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在小麦中对DON和NIV生产菌种的酶和基因表达反应的比较

Gabriela da Rocha Lemos Mendes¹, Paulo Cesar Pazdiora², Vivian Ebeling Viana¹

¹Centro de Genômica e Fitomelhoramento, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas 96010-900, Brazil.

|September 4, 2025

概括

小麦基因型对Fusarium头部病 (FHB) 的防御反应有所不同. 早期协调的生物化学和分子激活是小麦FHB耐药性的关键.

科学领域:

植物病理学
分子生物学
生物化学

背景情况:

菌头部病 (FHB) 严重影响小麦产量,由有毒的菌菌复合物种引起.
了解小麦对不同Fusarium化学型的防御机制对于开发耐药品种至关重要.

研究的目的:

在巴西小麦基因型中研究生物化学和分子防御反应,具有不同的FHB易感性.
用产生脱氧尼瓦醇 (DON) 和尼瓦醇 (NIV) 的Fusarium分离物接种后分析时间防御模式.

主要方法:

在接种后12至96小时内评估抗氧化剂和防御相关酶的时间模式.
通过转录组分析分析了ABC-转运体和Ca2+-ATPase的基因表达.
使用ANOVA来确定基因型,注射和时间对酶活动的显著影响.

主要成果:

基于小麦基因型和Fusarium化学型,酶活动和基因表达有显著差异.
Frontana显示高基底酶活性和增强的注射后反应;BRS 194显示延迟激活,特别是对DON生产者.
产生NIV的病原体增加了目标基因的多达18倍;产生DON的病原体诱导了Frontana的多达12倍.

结论:

小麦的生物化学和分子防御反应取决于基因型和病原体化学型.
早期和协调的防御机制激活对于有效的FHB耐药性至关重要.
这些发现为提高FHB耐药性的小麦品种提供了见解.

关键词:

美国 Fusarium与小麦的相互作用其他酵素的使用这些基因植物生物压力

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