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用于选择性检测和生物成像的水溶性化基光探针

Yang Yang¹, Yuxin Shao¹, Xiaoying Bao¹

¹Inner Mongolia Key Laboratory for the Natural Products Chemistry and Functional Molecular Synthesis; College of Chemistry and Materials Science, Inner Mongolia Minzu University, Tongliao 028000, PR China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|September 4, 2025

概括

一个新的光探测器,PTZMN,检测低化物 (ClO-),具有高灵敏度和选择性. 这种探测器是有效的环境监测和生物成像应用.

科学领域:

分析化学
化学传感器
生物医学成像

背景情况:

低化物 (ClO-) 是一个关键的反应性氧物种,参与生物过程和环境卫生.
对ClO-的选择性和敏感检测方法对于环境监测和生物研究至关重要.
现有的检测方法在复杂的生物基质中可能缺乏特异性,敏感性或适用性.

研究的目的:

为选择性和敏感的低化物检测开发一种基于酸的新型光探测器 (PTZMN).
研究PTZMN的传感机制和性能特征.
在环境样本和生物成像中证明PTZMN的实用性.

主要方法:

基于氨酸的光探针 (PTZMN) 的合成和表征.
谱学研究 (色度测量和光) 来评估探针对低酸盐的反应.
电子喷射电离质谱 (ESI-MS),1H核磁共振 (NMR) 和密度功能理论 (DFT) 用于解释机制.
在真实世界样本 (消毒剂,水) 和生物系统 (细胞成像,斑马鱼胚胎) 中应用探针.

主要成果:

在与低化物反应时,PTZMN在496nm处表现出明显的色度转移和"启动"光.
达到5.2 × 10−7mol/L的低检测极限,具有很好的线性 (R2 = 0.9956).
证实了低酸盐的氧化裂变,破坏了分子内电荷转移 (ICT).
在环境样本和测试带上证明了高选择性,抗干扰能力和成功应用.
在活的HeLa细胞和斑马鱼胚胎中成功应用了高分辨率光成像,没有可观察到的毒性.

结论:

PTZMN是一种高效的光探测器,用于选择性和敏感的低化物检测.
探测器的机制涉及氧化裂变的 imine 键,导致光启动反应.
对于环境分析,便携式检测设备和体内生物氧化还原成像,PTZMN具有显著的潜力.

关键词:

生物成像氨酸光探测器低化物类药物

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