基于四种菌株益生菌混合物的协生菌体内查及其治疗性结肠炎的潜力
Jiahao Liao1, Ying Guo2, Zhuoya Guo1
1Key Laboratory of Postharvest Preservation and Processing of Vegetables (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China; College of Animal Science, Shanxi Agricultural University, Taigu 030801, China.
结合果糖 (FOS) 和胰岛素 (INU) 与化合物益生菌的协生菌通过增强肠道屏障功能和减少炎症,有效缓解性结肠炎 (UC) 的症状. 这项研究突出了它们在UC治疗中的治疗潜力.
科学领域:
- 胃肠病学和微生物学
- 肠道微生物组研究
- 炎症性肠病治疗方法
背景情况:
- 性结肠炎是一种慢性炎症性肠病,治疗选择有限.
- 肠道微生物及其代谢物,如短链脂肪酸 (SCFA),对肠道健康起着至关重要的作用.
- 协生菌是益生菌和益生菌的组合, 是调节肠道环境的一个有希望的策略.
研究的目的:
- 在体外发酵模型和DSS诱导的UC小鼠模型上研究共生菌 (含有FOS或INU的化合物益生菌) 的治疗作用.
- 评估共生菌对益生菌增殖,SCFA生产,代谢调节和肠道屏障完整性的影响.
- 评估协生菌作为性结肠炎的辅助或替代疗法的潜力.
主要方法:
- 使用了体外发酵模型和Dextran硫酸 (DSS) 诱导的UC小鼠模型.
- 通过代谢学分析了益生菌生长,SCFA生产 (乙酸,酸,丁酸,异丁酸) 和肠道代谢物概况.
- 评估了UC症状的严重程度,结肠组织学,肠壁蛋白的表达 (Claudin-1,Occludin,ZO-1),以及细胞因子水平 (IL-1β,IL-6,TNF-α,IL-10).
主要成果:
- 协生菌 (CP+FOS,CP+INU) 显著增加了益生菌的生长和SCFA的产生.
- 协生菌改善了小鼠的UC症状,改善了体重,DAI得分和结肠长度,同时提高了脏指数.
- 协生菌保护了结肠密码,改善了屏障功能,恢复了紧密结蛋白,调节了促炎和抗炎细胞因子,并增加了SCFA水平.
结论:
- 复合益生菌与FOS或INU的协生组合显示出对性结肠炎的显著治疗潜力.
- 这些协生菌有效缓解UC症状,修复肠道屏障功能,调节炎症反应.
- 这些发现支持将共生药物作为一种有前途的辅助或替代性治疗策略.
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