在狐尾小米储存过程中对脂质降解和挥发性化合物的蛋白质学的洞察
Tong Wu1, Hang Zhou1, Zhihao Ma1
1College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China; National Center of Technology Innovation in Food Industry, China Agricultural University, China.
在储存期间,由于脂质分解和挥发性化合物变化,狐尾小米的质量下降. 蛋白质组学揭示了驱动这些变化的酶,影响了谷物的质地和味道.
科学领域:
- 农业科学
- 食品化学
- 生物化学
背景情况:
- 在储存过程中,牛尾小米的质量下降是一个重大问题.
- 导致老化质量下降的确切机制在很大程度上仍未被阐明.
研究的目的:
- 研究储存的狐尾小米中的脂质降解和挥发性化合物变化.
- 通过蛋白质组学来确定质量恶化的潜在机制.
主要方法:
- 气色谱-质谱 (GC/MS) 用于挥发性化合物的分析.
- 蛋白质组分析以确定蛋白质表达和代谢途径的变化.
- 对谷物的表面质地和脂肪酸含量的评估.
主要成果:
- 在储存30天后观察到脂肪酸含量增加和较粗的粒度.
- GC/MS显示酒精比例降低,而化物,酸和的含量增加.
- 蛋白质组学发现脂肪酸降解酶和过氧体相关蛋白质的升高,表明氧化应激增强.
结论:
- 上调的脂肪酸降解酶可能会导致短链有机酸的积累,影响粉和蛋白质的水解,导致谷物的微观结构发生变化.
- 增强的氧化应激有助于不和脂肪酸氧化为挥发性化合物.
- 这些分子和微观结构的变化共同解释了储存期间狐尾小米的质量下降.
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