通过调节肠道微生物的三甲代谢和AhR/NLRP3通路,Gastrodin可以改善性结肠炎
Dandan Zhang1, Jinlu Wu2, Hui Feng2
1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; TCM Prevention and Treatment of Metabolic and Chronic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610032, China; Key Laboratory of the Ministry of Education for Standardization of Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
通过调节肠道微生物群和代谢物,Gastrodin (GAS) 有效治疗性结肠炎. 这种传统中医药化合物通过酸受体 (AhR) /NLRP3途径抑制炎症,为UC提供新的治疗途径.
科学领域:
- 胃肠病学
- 药理学
- 微生物学
背景情况:
- 性结肠炎是一种慢性炎症性肠病,治疗选择有限.
- 来自Gastrodia elata的Gastrodin (GAS) 具有抗炎性质,但其在UC中的作用尚未被探索.
- 了解肠道微生物在UC病变中的作用对于开发新疗法至关重要.
研究的目的:
- 调查Gastrodin (GAS) 对DSS诱导的性结肠炎 (UC) 的保护作用.
- 阐明底层机制,重点关注肠道微生物群与代谢物的相互作用.
- 在气体介导UC处理中探索基碳化合物受体 (AhR) /NLRP3通路的作用.
主要方法:
- 在使用德克斯硫酸盐 (DSS) 的小鼠模型中诱导UC.
- 通过症状评估和组织学分析评估GAS疗效.
- 肠道微生物组成 (16S rRNA测序) 和便代谢学分析.
- 使用西式涂抹和MALDI-MSI对分子通路的研究.
- 通过便微生物体移植 (FMT) 验证微生物体依赖机制.
主要成果:
- GAS表明UC症状和结肠炎症的改善取决于剂量.
- GAS治疗显著增加了便和结肠中的酸衍生代谢物,包括酸 (Kyna),酸 (IAA),酸 (IAld) 和酸 (ILA).
- GAS激活了AhR/NLRP3通路,减少了结肠炎症,这些效应被证实是微生物群依赖的.
结论:
- 通过调节肠道微生物群和增加有益的甲酸代谢物,加斯特罗丁 (GAS) 可缓解性结肠炎 (UC).
- GAS通过抑制AhR/NLRP3炎症通路来发挥其保护作用.
- 这些发现突显了GAS作为UC的有前途治疗剂,利用传统医学和针对微生物群的策略.
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