在激活的巨细胞中,Arctoscopus japonicus的脂质通过eicosanoid合成途径抑制抗炎作用
Weerawan Rod-In1, Natchanok Talapphet2, Seok Kyu Jung3
1Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand; Center of Excellence in Research for Agricultural Biotechnology, Naresuan University, Phitsanulok 65000, Thailand.
Fish & shellfish immunology
|September 4, 2025
概括
通过抑制免疫细胞中的循环氧化酶 (COX),脂氧化酶 (LOX) 和细胞染色体P450 (CYP) 等关键酶,Arctoscopus japonicus脂质 (AJL) 减少炎症. 这种对eicosanoid通路的调节减轻了促炎反应,并促进了抗炎作用.
科学领域:
- 生物化学
- 免疫学
- 药理学
背景情况:
- 在炎症中,eicosanoids 是关键的脂质媒介,通过循环氧化酶 (COX),脂氧化酶 (LOX) 和细胞P450 (CYP) 途径合成.
- Arctoscopus japonicus脂质 (AJL) 显示出抗炎作用的潜力,但它们涉及eicosanoid合成的精确细胞机制需要阐明.
研究的目的:
- 调查AJL对LPS刺激的RAW264.7巨细胞中的eicosanoid合成途径的影响.
- 确定AJL如何调节促炎和抗炎细胞因子以及特定的eicosanoid代谢物.
主要方法:
- 使用LPS刺激RAW264.7细胞以诱导炎症.
- 评估了AJL治疗对细胞因子 (IL-1β,IL-6,TNF-α,IFN-γ,IL-12,TGF-β,IL-10) 的影响.
- 测量了COX,LOX和CYP途径代谢物 (TXA2,PG,LTB4,EET) 的水平.
- 分析了关键酶 (COX-1,COX-2,5-LOX,CYP4A11) 和CD86的基因和蛋白质表达.
主要成果:
- AJL显著降低了促炎细胞因子 (IL-1β,IL-6,TNF-α) 和增加了抗炎细胞因子 (TGF-β,IL-10).
- AJL抑制了主要的eicosanoid的产生,包括血栓素A2,前列腺素,白血素B4和环氧酸.
- 在LPS刺激细胞中,AJL降低了COX-1,COX-2,5-LOX,CYP4A11和CD86的表达.
结论:
- 通过调节eicosanoid合成途径,AJL具有抗炎作用.
- 抑制参与COX,LOX和CYP通路的关键酶,从而减少炎症媒介的产生.
- AJL降低促炎标志物和酶的能力支持其在炎症状况中的治疗潜力.
相关概念视频
01:38Inflammation
55.0K
Overview
55.0K
01:20The JAK-STAT Signaling Pathway
9.2K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
9.2K
01:31Lipid-derived Compounds in the Human Body
5.2K
Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin,...
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin,...
5.2K
01:28Inflammatory Response
6.9K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
6.9K
01:29Biosynthesis of Lipids
91
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
91
02:26NF-κB-dependent Signaling Pathway
7.8K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.8K