通过诱导M2巨细胞偏离,增强了tsRNA-10105的迁移体 中介性肝细胞癌 免疫抑制微环境
Ruiyao Zhou1, Limin Pan2, Yu Zeng3
1Department of General Surgery, Ruian People's Hospital, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
Experimental cell research
|September 4, 2025
概括
通过诱导免疫抑制M2巨细胞来促进肝癌细胞的瘤生长. 在迁移体中抑制tsRNA-10105可能提供新的肝癌免疫疗法策略.
科学领域:
- 免疫学
- 癌症学
- 分子生物学
背景情况:
- 肝细胞癌 (HCC) 具有阻碍治疗的免疫抑制瘤微环境.
- 新型细胞外囊泡 (Migrasomes) 参与细胞间通信,但它们在HCC免疫力中的作用尚未被探索.
研究的目的:
- 探讨迁移体在HCC免疫微环境中的作用.
- 确定迁移体是否会影响巨细胞极化和HCC的进展.
- 识别涉及HCC免疫调节的迁移体中的关键分子.
主要方法:
- 在HCC组织上进行免疫光检测,以检测迁移体和M2标记物.
- 从HCC细胞中分离和表征迁移体 (电子显微镜,免疫光,西斑).
- 在体外和体内测试以评估对巨细胞和HCC细胞的迁移体影响.
- 小RNA测序 (RNA-seq) 来识别关键的tsRNA.
主要成果:
- 在HCC组织中观察到偏移体和M2巨标记的升高.
- 在巨细胞中诱导M2极化,抑制M1标记物.
- 在体内治疗的巨细胞增强了HCC细胞的增殖,迁移,侵袭和瘤生长/转移.
- 在HCC患者的血清中发现高的tsRNA-10105;其抑制阻断了M2极化诱导.
结论:
- 通过诱导M2巨分化,转基因衍生的tsRNA-10105促进了HCC的进展.
- 这种机制有助于HCC的免疫抑制微环境.
- 在肝癌免疫治疗中,向tRNA-10105是潜在的治疗策略.
更多相关视频
相关概念视频
02:17The Tumor Microenvironment
6.3K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.3K
01:22MicroRNAs
3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K