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Updated: Sep 9, 2025

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon
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Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

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一个实证偏差力常数,以最大限度地减少冷-电磁灵活的装配精细化

Daisuke Matsuoka1, Yuji Sugita2,3, Takaharu Mori2,4

  • 1RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.

Journal of chemical information and modeling
|September 4, 2025
PubMed
概括

我们建议使用冷电子显微镜 (cryo-EM) 数据对蛋白质结构进行细化. 这一准则有助于最大限度地减少灵活的装配,提高模型的可靠性.

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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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