马术运动中的苏泽特里金:优化提取和LC-HRMS检测策略
Meleparappil Muhammed Ajeebsanu1, Shino Ann Koshy1, Abdul Khader Karakka Kal1
1Equine Forensic Unit, Central Veterinary Research Laboratory, Dubai, UAE.
Rapid communications in mass spectrometry : RCM
|September 4, 2025
概括
一种新的方法可靠地检测用于兴奋剂控制的马类样本中的suzetrigine,一种疼痛治疗药物. 这种经过验证的程序确保了准确的识别,防止了体育中的滥用.
科学领域:
- 药理学
- 分析化学
- 运动科学
背景情况:
- 苏丁是一种NaV1. 8阻断剂,可缓解疼痛,没有中枢神经系统的副作用或成风险.
- 人们担心在运动中滥用suzetrigine来提高表现.
- 可靠的检测方法对于反兴奋剂工作至关重要.
研究的目的:
- 在马类生物样本中开发和验证检测suzetrigine的分析方法.
- 为兴奋剂控制实验室建立一个强有力的协议.
- 为了应对在体育领域滥用苏丁的新挑战.
主要方法:
- 对色谱列,移动相和电离模式进行系统测试.
- 固态提取 (SPE),液态提取 (LLE) 和稀释注入技术的评估.
- 使用高分辨率质谱测试方法验证.
主要成果:
- 在 AQUA C18 列提供最佳分离.
- 优化的LLE显示出更高的恢复和更低的检测极限.
- 经过验证的方法显示出出色的灵敏度,可重复性和稳定性.
结论:
- 有一种验证的分析方法可用于检测用于兴奋剂控制的马类样本中的suzetrigine.
- 该协议作为更广泛的反兴奋剂应用的框架.
- 这有助于监测可能在体育中被滥用的新兴物质.
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