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Updated: Sep 9, 2025

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口腔平菌的lncRNA/circRNAs-miRNA-mRNA网络的综合分析

Muyang Zhang¹, Limin Miao², Huyan Chen³

¹Department of Stomatology, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.

Journal of inflammation research

|September 5, 2025

概括

这项研究确定了COTL1,OAS2,HLA-A和HLA-DPA1等关键基因参与口腔平 (OLP),并揭示了潜在的OLP诊断和治疗的监管网络.

科学领域:

分子生物学
基因组学
免疫学

背景情况:

口腔平 (OLP) 是一种T细胞介导的口腔粘膜炎症,其分子机制尚不清楚.
识别关键的分子参与者对于了解OLP病变和开发向疗法至关重要.

研究的目的:

使用集成的转录和蛋白质基因选与OLP相关的枢纽基因.
构建竞争的内源性RNA (ceRNA) 网络以阐明疾病机制.
评估OLP中发现的枢纽基因的诊断潜力.

主要方法:

来自OLP患者和健康对照者的转录和蛋白质组数据的综合分析.
差异基因表达分析,蛋白与蛋白相互作用网络构建和加权基因共表达网络分析.
核基因的鉴定,ceRNA网络的构建和qPCR验证.

主要成果:

确定了103个差异表达的基因 (DEG),这些基因在抗原处理和呈现等途径中得到丰富.
鉴定出COTL1,OAS2,HLA-A和HLA-DPA1是具有诊断功效的枢纽基因 (AUC> 0. 7).
构建并验证了涉及LncRNA MIR155HG,hsa- miR-1233-5p,COTL1和OAS2的ceRNA网络.

结论:

综合多组数据揭示了OLP的关键分子机制.
已识别的枢纽基因和ceRNA网络为OLP提供了潜在的诊断生物标志物和治疗点.
对circRNA/lncRNA-miRNA-mRNA网络的进一步研究可以促进OLP的理解和治疗.

关键词:

竞争的内源性RNA 非编码RNA 在口腔的平坦权重基因共同表达网络分析

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