评估脂肪体载体的生物分布,稳定性和清除的多模式方法
Cayla A Wood1,2, Claire E Jones2, Ananthakrishnan Soundaram Jeevarathinam2
1Department of Radiology, The University of New Mexico, 1 University of New Mexico, Albuquerque, NM 87131, United States.
这项研究引入了一种多式成像技术,使用脂质体封装的J-聚合绿色 (Lipo-JICG) 来追踪脂质体载体. 该方法有效评估生物分布和清除,这对于开发有针对性的药物输送和诊断系统至关重要.
科学领域:
- 纳米医学和药物输送
- 生物医学成像
- 药物动力学
背景情况:
- 脂质体载体对于向药物输送至关重要,但评估它们的体内行为仍然具有挑战性.
- 开发实时监测脂质体生物分布,稳定性和清除的方法对于推进诊断和治疗应用至关重要.
- 这项试点研究解决了改善脂肪体载体体体内评估的需要.
研究的目的:
- 开发和验证一种多式成像方法,以评估体内脂肪体载体的行为.
- 通过光声成像和冷光学断层扫描评估脂质体封装的J-聚合ICG (Lipo-JICG).
- 研究Lipo-JICG的生物分布,稳定性和清除动力学.
主要方法:
- 在实验室中制备和鉴定了脂质体封装的J-聚合ICG (Lipo- JICG).
- 在体内光声成像 (PA) 用于监测Lipo- JICG和单体ICG信号.
- 使用活体冷光学断层扫描 (CFT) 来测量ICG光分布.
主要成果:
- 在体外研究表明,J聚合会灭ICG光,并改变吸收率.
- 在体内PA成像显示Lipo- JICG信号在肝脏和脏达到30分钟的峰值.
- 在6天的时间内,CFT显示了Lipo- JICG分解和肝脏清除.
结论:
- 多模式成像方法有效评估脂肪体载体的生物分布和清除.
- 脂JICG显示了特定的生物分布模式和逐渐清除.
- 这种方法在临床前模型中对脂肪体载体的评估具有前景.
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