同轴电对齐核心外聚 (乙烯氧化物-聚 ((l-乳化物-co-糖化物)) 纳米纤维封装神经生长因子的过程优化和释放建模
Bhoomija Hariprasad1, Mohammadjavad Eslamian1, Nihir Patel1
1Department of Biomedical Engineering, University of Houston.
概括
这项研究优化了同轴电旋转,以创建对齐的神经生长因子 (NGF) 装载的纳米纤维用于神经再生. 该过程实现了最小的纤维直径和狭窄的尺寸分布,从而实现了受控的蛋白质释放.
科学领域:
- 生物材料科学
- 神经工程
- 聚合物化学
背景情况:
- 神经再生策略受益于聚合物囊中的蛋白质提供的地形和生化线索.
- 同轴电是一种嵌入生物活性剂在核心纳米纤维的关键技术,以控制释放.
研究的目的:
- 系统地研究同轴电旋参数,以优化带有神经生长因子 (NGF) 的对齐的聚乙烯氧化物-聚乙烯乳化物-同糖化物纳米纤维.
- 为了实现最大限度的纤维直径和狭窄的尺寸分布,以增强神经再生应用.
主要方法:
- 使用盒式设计 (BBD) 来设计实验方法以确定最佳的电旋参数.
- 进行回归分析以确定诸如流速,收集器速度,电压和距离等参数对纳米纤维特性的影响.
- 使用优化的核心外纳米纤维特征NGF释放动力学.
主要成果:
- 通过特定的参数设置实现了纤维直径 (323 nm) 和尺寸分布 (2.37%):内流速 (0.33 mL/h),外流速 (2 mL/h),收集器转速 (500 rpm),电压 (17 kV) 和距离 (10 cm).
- 内部流量,收集器距离和电压显著影响了纤维直径,而收集器速度对于尺寸分布至关重要.
- NGF释放呈现双相性行为:最初的突发释放 (约81%在8小时内) 随后持续释放 (约13%在两周内),符合迈凯利斯- 门模型.
结论:
- 建立了一种高效和可持续的方法,用于制造具有控制尺寸的NGF加载,对齐的核心外纳米纤维.
- 通过提供精确的地形和生化线索, 优化的纳米纤维具有推进神经再生疗法的巨大潜力.
- 双相释放动力学表明PEO核心溶解和PLGA降解机制,提供可调节的药物输送配置.
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