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Caiyi Zhang¹, Jie Zhao¹, Keqing Pan¹

¹Department of Stomatology, the Affiliated Hospital of Qingdao University, Qingdao, China, School of Stomatology, Qingdao University, Qingdao, China.

Frontiers in immunology

|September 5, 2025

概括

相关的基因与慢性牙周炎有关. 这些基因,特别是涉及纤维细胞和巨细胞,显示出诊断潜力,并可能驱动疾病的进展.

科学领域:

口腔生物学
细胞死亡途径
基因组学

背景情况:

慢性牙周炎 (CAP) 是一种常见的口腔炎症,其潜在机制尚不清楚.
一种新的细胞死亡途径cuproptosis是CAP发展的潜在因素.

研究的目的:

为了确定与CAP相关的cuproptosis相关基因 (CRG).
研究CRG在CAP细胞相互作用中的作用.
评估CRG在CAP中的诊断潜力.

主要方法:

综合基因表达综合 (GEO) 数据集用于CAP和健康对照 (HC).
分析单细胞RNA测序 (scRNA-seq) 数据以评估细胞群变异.
使用受体操作特征 (ROC) 曲线,qRT-PCR和免疫组织化学 (IHC) 进行验证.

主要成果:

在CAP中确定了8个差异表达的CRG (DE-CRG).
在CAP纤维细胞和巨细胞中发现了涉及CRG的显著变化和细胞间通信.
通过qRT-PCR和IHC在CAP组织中确认DE-CRG的表达,显示出强大的诊断潜力.

结论:

在CAP的发病过程中涉及CRG.
涉及CRG的COL4A1- 纤维细胞和APOE- 巨相互作用可能会促进CAP的亡.
这些CRG代表了CAP的潜在诊断生物标志物.

关键词:

细胞间的通信慢性牙周炎铜平衡的失衡这种疾病的病因单细胞RNA测序

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