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Updated: Sep 9, 2025

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

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Published on: March 12, 2021

整合性批量和单细胞转录组分析确定了与迁移体相关的lncRNA特征,可预测清细胞瘤的预后和免疫格局

Junlin Shen¹, Chun Wang¹, Mingpeng Zhang¹

¹Department of Urology, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin, China.

Frontiers in immunology

|September 5, 2025

概括

这项研究发现了基于清细胞癌的长非编码RNAs (lncRNAs) 的新诊断特征. 这种特征预测患者的存活率,并揭示不同的瘤特征,为ccRCC提供新的治疗策略.

科学领域:

癌症学
分子生物学
基因组学

背景情况:

清细胞细胞癌 (ccRCC) 的复发和转移率很高,导致预后不佳.
迁移体和长非编码RNA (lncRNAs) 与瘤进展有关,但它们在ccRCC中的综合预后作用尚不清楚.

研究的目的:

识别与迁移体相关的 lncRNA 并构建ccRCC的预后风险特征.
评估该特征与整体存活率,瘤突变负担,瘤微环境,免疫逃避和药物敏感性的关联.
使用单细胞转录学探索细胞类型特异表达和细胞间通信网络.

主要方法:

使用癌症基因组图谱 (TCGA) 数据集来识别与迁移组相关的lncRNA并构建预后模型.
评估了风险特征与临床/基因特征之间的相关性,包括瘤微环境 (TME) 和免疫逃避.
进行单细胞转录组分析和体外实验以验证关键的 lncRNA 功能.

主要成果:

构建的风险特征显著地将ccRCC患者分为高风险和低风险组,具有不同的生存结果.
高风险患者的瘤突变负担 (TMB) 高,免疫规避能力强.
单细胞分析显示了与特征相关的细胞异质性和TME特征,FOXD2-AS1抑制了瘤的进展.

结论:

一种新的与迁移体相关的 lncRNA 风险特征对ccRCC具有显著的预后和治疗潜力.
这种特征有效地捕捉了ccRCC的独特免疫,基因组和药理特征.
关键的 lncRNA 可能通过迁移体介导途径驱动ccRCC的进展,需要进一步的机制研究.

关键词:

清细胞细胞癌药物敏感性转移组预后签名单细胞RNA测序瘤免疫微环境瘤突变负担

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