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相关概念视频

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Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
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相关实验视频

Updated: May 3, 2026

An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma
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使用猪甲状腺蛋白构建最佳的自身免疫甲状腺炎小鼠模型

Ke Liu1, Pei Zhang2, Zi-Shan Jin3

  • 1Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

Frontiers in immunology
|September 5, 2025
PubMed
概括
此摘要是机器生成的。

开发一种针对自身免疫性甲状腺炎 (AIT) 的最佳动物模型需要对NOD/LtJ小鼠的免疫策略进行比较. 高剂量的抗原和频繁的注射有效诱导了AIT,为AIT的发病和治疗研究铺平了道路.

关键词:
在NOD/LtJ小鼠中动物模型的构造自免疫性甲状腺炎实验性自身免疫性甲状腺炎分子机制

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科学领域:

  • 免疫学
  • 内分泌学
  • 动物模型

背景情况:

  • 自体免疫甲状腺炎 (AIT) 是一个越来越令人担忧的疾病,没有标准化的动物模型.
  • 了解AIT病变和开发向免疫疗法需要一个可靠的模型.

研究的目的:

  • 系统地比较不同的免疫条件,以开发最佳的NOD/LtJ小鼠AIT模型.
  • 阐明不同抗原剂量,频率和施用途径的病理和免疫效应.

主要方法:

  • 在不同剂量和频率下,NOD/ LtJ小鼠接受皮下 (SC) 或静脉注射 (IV) 猪甲状腺蛋白 (pTg) 免疫接种.
  • 分析了甲状腺组织病理,血清自身抗体 (TPO- Ab,TG- Ab),细胞因子,T辅助17 (Th17) /调节性T (Treg) 细胞以及炎症组分 (NLRP3,Caspase-1).

主要成果:

  • 在SC和IV组中,高剂量 (200μg) pTg与三次免疫诱导严重的甲状腺炎,卵泡破坏和自抗体升高.
  • 这种方案激活了Th1/ Th17细胞因子和增加了Treg细胞,增强了甲状腺NLRP3炎症酶的激活.
  • 静脉注射显示出更高的抗体产生和炎症酶激活,而静脉注射导致更明显的组织炎症.

结论:

  • 高剂量抗原 (200μg pTg) 和三次免疫,特别是通过尾静脉注射,有效地模拟了NOD/LtJ小鼠中的AIT.
  • 这种优化的协议提高了AIT研究的实验效率和可重复性.
  • 这些发现为研究AIT病变和评估新型免疫疗法提供了坚实的基础.