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Updated: Sep 9, 2025

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
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Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

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一个集成的酶和计算管道来量化非目标基数编辑

Alexander G McFarland, Soon-Keat Ooi, Davin Tafuri

    bioRxiv : the preprint server for biology
    |September 5, 2025
    PubMed
    概括

    一种新的方法通过标记双链DNA断裂来识别基因编辑导致的意外DNA变化. 这种方法有助于监测患者细胞的非目标编辑,提高基因编辑疗法的安全性.

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    One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
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