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Updated: Sep 9, 2025

Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder ALLIGATOR
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Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder ALLIGATOR

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通过专家指导的深度学习推进Luciferase活动和稳定性

Spencer Gardiner, Joseph Talley, Christopher Haynie

    bioRxiv : the preprint server for biology
    |September 5, 2025
    PubMed
    概括

    在高温下改造的NanoLuc luciferase (NLuc) 变种显示出更好的热稳定性和活性. 这项突破利用了混合深度学习和理性设计方法,

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    Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

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    Improving Translational Accuracy

    Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
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