Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

121
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
121
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

208
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
208
CRISPR01:59

CRISPR

52.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.8K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.1K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Dexamethasone restores blood-brain barrier integrity in an in vitro heatstroke model.

PloS one·2026
Same author

A controlled longitudinal study clarifies the contours of pediatric long COVID.

Pediatric research·2026
Same author

Placental Pathogens Associated With Adverse Maternal and Neonatal Outcomes.

Open forum infectious diseases·2026
Same author

A cross-strain CRISPRi sgRNA library for <i>Streptococcus agalactiae</i>.

Microbiology resource announcements·2026
Same author

Real-World Clinical Datasets in Practice: Applications for Learners, Clinician-Educators, and Health Services Teams.

Clinical and translational science·2026
Same author

Analysis of the <i>Salmonella enterica</i> serovar Typhimurium Chitobiose ( <i>chb</i> ) Operon.

microPublication biology·2026

相关实验视频

Updated: Sep 9, 2025

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
14:49

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

5.2K

在Streptococcus agalactiae多部位序列17型菌株中的CRISPR干扰

William D Cutts, Aidan W Flanagan, Brice Gorman

    bioRxiv : the preprint server for biology
    |September 5, 2025
    PubMed
    概括

    研究人员为B组链球菌 (GBS) ST-17菌株开发了CRISPR干扰系统. 这种工具可以通过向基因敲除来研究GBS脑膜炎在血脑屏障上的致病性.

    更多相关视频

    Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
    10:46

    Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

    Published on: October 18, 2022

    1.8K
    Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
    05:48

    Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

    Published on: March 16, 2022

    2.7K

    相关实验视频

    Last Updated: Sep 9, 2025

    Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
    14:49

    Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

    Published on: August 14, 2021

    5.2K
    Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
    10:46

    Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

    Published on: October 18, 2022

    1.8K
    Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
    05:48

    Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

    Published on: March 16, 2022

    2.7K

    科学领域:

    • 微生物学
    • 遗传学
    • 传染性疾病

    背景情况:

    • B型链球菌 (GBS) 是新生儿细菌性脑膜炎的主要原因.
    • 像COH1一样,高病毒性血清型III,序列型17 (ST-17) 的GBS菌株与严重的新生儿疾病有很强的联系.
    • 对ST-17GBS菌株进行基因操作是很困难的,这阻碍了对毒性因素的研究.

    研究的目的:

    • 在ST-17 GBS COH1菌株中开发CRISPR干扰 (CRISPRi) 系统.
    • 为了使功能基因组学和高通量查GBS毒性因素.
    • 促进血脑屏障的GBS病变研究.

    主要方法:

    • 在COH1 GBS菌株中使用催化无活化Cas9 (dCas9) 的CRISPR干扰系统的开发.
    • 使用血液溶解试验,qPCR和使用人脑内皮细胞的体外感染模型来证实系统的有效性.
    • 针对性打击关键病毒性基因,包括pilA,srr2和iagA.

    主要成果:

    • 在ST-17 GBS COH1中成功实现可调整的CRISPRi系统.
    • 证明了基本的GBS毒性基因的表型破坏.
    • 减少了血脑屏障中的细菌粘附,入侵和炎症反应.

    结论:

    • 开发的CRISPRi系统为ST-17 GBS的快速基因淘汰提供了一个多功能平台.
    • 这种工具克服了COH1的先前基因操纵挑战.
    • 能够对血脑屏障中的GBS病变和宿主病原体相互作用进行先进的研究.